Amino acid composition of cytoplasmic yeast pyruvate decarboxylase
نویسندگان
چکیده
منابع مشابه
Regulating Pyruvate Decarboxylase Synthesis in Yeast
Mutants lacking pyruvate decarboxylase cannot grow on glucose. We have isolated three different complementation groups of extragenic suppressors that suppress mutations in pdc2, a regulatory locus required for the synthesis of the glycolytic enzyme pyruvate decarboxylase. The most frequent of these is a recessive mutation in the structural gene PFKl of the soluble phosphofructokinase. The other...
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The recent finding (1, 2) that part of the enolase molecule can be removed by digestion with leucine aminopeptidase or carboxypeptidase without change in enzymic activity has prompted a detailed study of the relation between structure and activity in this enzyme. As a basis for such investigations it is necessary to have a knowledge of the principal features of enolase structure; the amino acid...
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Six different pyruvate decarboxylase mutants of Saccharomyces cerevisiae were isolated. They belong to two unlinked complementation groups. Evidence is presented that one group is affected in a structural gene. The fact that five of the six mutants had residual pyruvate decarboxylase activity provided the opportunity for an intensive physiological characterization. It was shown that the loss of...
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To study the mechanism of re-activation of Zymomonas mobilis pyruvate decarboxylase apoenzyme by its cofactors thiamin diphosphate and Mg2", cofactor-free enzyme was prepared by dialysis against I mM-dipicolinic acid at pH 8.2. This apoenzyme was then used in a series of experiments that included determination of: (a) the affinity towards one cofactor when the other was present at saturating co...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1969
ISSN: 0014-5793
DOI: 10.1016/0014-5793(69)80254-1